Coding

Part:BBa_K2656025:Design

Designed by: Adrián Requena Gutiérrez   Group: iGEM18_Valencia_UPV   (2018-09-23)


BSMT1 Coding Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 664
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It has been designed changing the T in 207 for a C to delete the BsaI site and adding to the CDS sequence: GCGCCGTCTCGCTCGA upstream and GCTTTGAGCGAGACGGCGC downstream. These sequences allow the assembly of the part into BBa_P10500 with a Golden Gate reaction using BsmbI endonuclease.


Source

The original sequence has been obtained from the Registry of Standard Biological Parts and it has been synthetized by IDT.

References