RNA
Part:BBa_K2654002:Design
Designed by: Alexandre Jolly Group: iGEM18_UCLouvain (2018-10-09)
sgRNA with a switchable crRNA sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 12
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 640
Illegal AgeI site found at 752 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Verify that the four igem restriction sites were absent of the biobrick. We suppressed the DAM sequences to avoid potential methylations. Verification of the presence of a functional terminator. Verification that the sequence is a correct sgRNA according to the literature. In consequence, all the sequences were modified to correspond to the Biobrick standards and the iGEM requirements.
Source
A strong promoter J23119 produce the sgRNA and RFP