Plasmid_Backbone

Part:BBa_K2649002

Designed by: Alexandrea Pouliot   Group: iGEM18_MIT   (2018-10-12)


RFP SB1C3 w/ SapI

We want to make a vector that will allow us to construct L0s for parts in our lab using the same backbone. To do so, we will modify the RFP that iGEM gives to add SapI sites (AGC cut at beginning, GTG at end, according to the Weiss Lab sites) as well as some of the BioBrick sites (full prefix at beginning, SpeI only at end). Then, we can attach it to the linearized pSB1C3 from iGEM and use it. From this, we can construct a ton of L0s out of Lab parts.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 247
    Illegal XbaI site found at 220
    Illegal PstI site found at 208
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 247
    Illegal PstI site found at 208
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 247
    Illegal BamHI site found at 226
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 247
    Illegal XbaI site found at 220
    Illegal PstI site found at 208
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 247
    Illegal XbaI site found at 220
    Illegal PstI site found at 208
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 598


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