Plasmid_Backbone
Part:BBa_K2649002
Designed by: Alexandrea Pouliot Group: iGEM18_MIT (2018-10-12)
RFP SB1C3 w/ SapI
We want to make a vector that will allow us to construct L0s for parts in our lab using the same backbone. To do so, we will modify the RFP that iGEM gives to add SapI sites (AGC cut at beginning, GTG at end, according to the Weiss Lab sites) as well as some of the BioBrick sites (full prefix at beginning, SpeI only at end). Then, we can attach it to the linearized pSB1C3 from iGEM and use it. From this, we can construct a ton of L0s out of Lab parts.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 247
Illegal XbaI site found at 220
Illegal PstI site found at 208 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 247
Illegal PstI site found at 208 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 247
Illegal BamHI site found at 226 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 247
Illegal XbaI site found at 220
Illegal PstI site found at 208 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 247
Illegal XbaI site found at 220
Illegal PstI site found at 208 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 1
Illegal BsaI.rc site found at 598
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Categories
Parameters
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