Coding

Part:BBa_K2647001:Design

Designed by: Jessica Koski   Group: iGEM18_Aalto-Helsinki   (2018-10-08)


mRFP linked to spider silk repeats and keratin binding domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 715
    Illegal BsaI.rc site found at 1534
    Illegal SapI.rc site found at 1997


Design Notes

codon optimised to E.coli


Source

Its components BBa_E1010, BBa_K2647000 and BBa_K2467002

References