Part:BBa_K2644010
J23104+ArsR
It's a combinition of promoter J23104, RBS B0034 and gene ArsR, which is sensitive to As(III).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 198
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage
It is a combined part. J23014 is a constitutive promoter in E.coli. ArsR is a regulator protein, which can bind asrR promoter and be used as a biological strategy to detect arsenic ions .When the part works, the cell can express Arsr protein and its ability to bind asrR promoter related to the concentration of arsenic ions so that the cell has the function of arsenic ions detection.
Biology
ArsR protein is a repressor protein. It can bind the asrR promoter to prevent downstream gene expression and leave the promoter if it senses arsenic ions. To make E.coli. cell achieve arsenic ions detection, we need make the part connect with asrR promoter and report protein sequence, such as fluorescent protein. So we can determine arsenic ions concentration by measuring fluorescence intensity.
Reference
[1] Davide Merulla1, Nina Buffi2, Siham Beggah1, Fre ́ de ́ ric Truffer3, Martial Geiser3, Philippe Renaud2 and Jan Roelof van der Meer1. Bioreporters and biosensors for arsenic detection. Biotechnological solutions for a world-wide pollution problem. [J].Current Opinion in Biotechnology 2013, 24:534–541
| None |