Part:BBa_K2644007
smURFP
A gene for fluorescent protein smURFP
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage
A sequence for fluorescent protein small Ultra-Red FP (smURFP). SmURFP can incorporate a more membrane-permeant BV analog, making smURFP fluorescence in situ comparable to FPs from jellyfish/coral.SmURFP has higher exciting and receiving light and its detecton is less affected by cellular background expresson compared with cyan, green, yellow and orange FPs. In our group, it has been used as report protein for its excellent properties to detect arsenic ions.
Biology
In order to fluorescence, smURFP must be combined with biliverdin (BV) .To make it come true,we construct the co-expression system.the gene of fluorescent protein---smURFP and the gene of the precursor of biliverdin---HO-1 should be connected to the same expression vector and then transferred to our target bacteria. The precursor of biliverdin will be transferred to biliverdin through a series of conversion, and then fluorescent protein will combine with biliverdin directly in our target bacteria and glow in the bacteria. In our arsenic ions detective circuit, ArsR repressor prontein will be removed when existing arsenic ions in vitro so that smURFP is able to expression.
Reference
[1] John V Frangioni. In vivo near-infrared fluorescence imaging. [J].Current Opinion in Chemical Biology 2003, 7:626–634 [2] Rodriguez EA,Tran GN , Gross LA, et al. A far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein .[J].NATURE METHODS,2016:763-769.
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