DNA

Part:BBa_K2644004

Designed by: Yiran Cheng   Group: iGEM18_TJU_China   (2018-07-30)


ArsR operator

An operator to control the expression of smURFP by binding ArsR protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage

ArsR operator is consist of an ArsR promoter and binding site which the ArsR repressor protein binds to. When combined with ArsR, the gene behind can not be transcribed. Conversely, when the ArsR dissociates from the promoter in the presence of arsenite, the gene behind can work as normal.

Biology

In order to control the expression of target gene by changing the concentration of As(III), we design a operator composing of ArsR promoter and ArsR repressor protein binding site.

Reference

[1]Davide Merulla1, Nina Buffi2, Siham Beggah1, Fre ́ de ́ ric Truffer3, Martial Geiser3, Philippe Renaud2 and Jan Roelof van der Meer1. Bioreporters and biosensors for arsenic detection. Biotechnological solutions for a world-wide pollution problem. [J] Current Opinion in Biotechnology 2013, 24:534–541

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