Coding
Part:BBa_K2638999:Design
Designed by: Vanessa Krämer, Matthias Otto Group: iGEM18_Bielefeld-CeBiTec (2018-08-31)
Mutated Human Ferritin Heavy Chain
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 184
Design Notes
Codon optimization for E. coli Removal of restriction sites over
Source
The sequence of the Human Ferritin Heavy Chain gene was codon optimized for E. coli and mutaded.
Directing Noble Metal Ion Chemistry within a Designed Ferritin Protein, Christopher A. Butts, Joe Swift, Seung-gu Kang, Luigi Di Costanzo, David W. Christianson, Jeffery G. Saven, and Ivan J. Dmochowski Biochemistry 2008 47 (48), 12729-12739 DOI: 10.1021/bi8016735