Coding

Part:BBa_K2638999:Design

Designed by: Vanessa Krämer, Matthias Otto   Group: iGEM18_Bielefeld-CeBiTec   (2018-08-31)


Mutated Human Ferritin Heavy Chain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 184


Design Notes

Codon optimization for E. coli Removal of restriction sites over


Source

The sequence of the Human Ferritin Heavy Chain gene was codon optimized for E. coli and mutaded.

Directing Noble Metal Ion Chemistry within a Designed Ferritin Protein, Christopher A. Butts, Joe Swift, Seung-gu Kang, Luigi Di Costanzo, David W. Christianson, Jeffery G. Saven, and Ivan J. Dmochowski Biochemistry 2008 47 (48), 12729-12739 DOI: 10.1021/bi8016735

References