Generator

Part:BBa_K2638759:Design

Designed by: Antonin Lenzen   Group: iGEM18_Bielefeld-CeBiTec   (2018-10-10)


Seamless siRNA Expression Vektor with an Hfq binding tag and OmpA 5'-UTR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 1565
    Illegal XhoI site found at 1864
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1869
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1771
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

The parts was constructed without to be able to express the siRNA without extra bases.


Source

Gene Syntheses, 2018 distribution Plates.

References