Generator
Part:BBa_K2638702:Design
Designed by: Antonin Lenzen Group: iGEM18_Bielefeld-CeBiTec (2018-09-02)
Seamless siRNA Expression Vektor for Hfq binding siRNAs
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal XhoI site found at 1448
Illegal XhoI site found at 1747 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1752
Illegal AgeI site found at 979
Illegal AgeI site found at 1654 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
The Golden Gate Casette was use to allow seamless cloning of siRNAs into the Vektor.
Source
Escherichia coli K12 genome,BBa_J61048 and BBa_I0500 from the 2018 Distribution Plates, Gene Synthesis.