Generator

Part:BBa_K2638702:Design

Designed by: Antonin Lenzen   Group: iGEM18_Bielefeld-CeBiTec   (2018-09-02)


Seamless siRNA Expression Vektor for Hfq binding siRNAs


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 1448
    Illegal XhoI site found at 1747
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1752
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1654
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

The Golden Gate Casette was use to allow seamless cloning of siRNAs into the Vektor.


Source

Escherichia coli K12 genome,BBa_J61048 and BBa_I0500 from the 2018 Distribution Plates, Gene Synthesis.

References