Coding

Part:BBa_K2638211

Designed by: Jakob Zubek   Group: iGEM18_Bielefeld-CeBiTec   (2018-09-02)


Tn501MerA (S558H, S559H)

Tn501MerA (BBa_K2638210) with two amino acid mutations from C to H at the positions 558 and 559. Those cysteines were important for the transport of the mercury into the active side.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 195
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1311
    Illegal AgeI site found at 1081
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1387


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Categories
Parameters
None