Composite
Part:BBa_K2638205:Design
Designed by: Jakob Zubek Group: iGEM18_Bielefeld-CeBiTec (2018-09-02)
OprC (TonB dependent copper transport porin, BBa_K2638200) with CueR and copAP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 562
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 562 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 562
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 562
Illegal NgoMIV site found at 824
Illegal NgoMIV site found at 1155
Illegal NgoMIV site found at 1359
Illegal NgoMIV site found at 2403
Illegal NgoMIV site found at 2604
Illegal AgeI site found at 818 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 475
Design Notes
This biobrick is a positive feedback loop, which causes the cell to take up a lethal dose of copper.
Source
Escherichia Coli K12 and Pseudomonas brassicacearum 3Re2-7 13