Composite

Part:BBa_K2638205

Designed by: Jakob Zubek   Group: iGEM18_Bielefeld-CeBiTec   (2018-09-02)


OprC (TonB dependent copper transport porin, BBa_K2638200) with CueR and copAP

OprC (BBa_K2638200) is an outer membrane porin which binds and transports specifically copper. It belongs to the the superfamily of the TonB-dependent transporters. This variant of OprC was taken from Pseudomonas brassicacearum 3Re2-7 13, a not yet published strain.

This composite Part contains additionally a CueR (BBa_K17583204) and the CueR binding promoter copAP (BBa_K2638230).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 562
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 562
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 562
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 562
    Illegal NgoMIV site found at 824
    Illegal NgoMIV site found at 1155
    Illegal NgoMIV site found at 1359
    Illegal NgoMIV site found at 2403
    Illegal NgoMIV site found at 2604
    Illegal AgeI site found at 818
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 475


[edit]
Categories
Parameters
None