Composite

Part:BBa_K2638201

Designed by: Jakob Zubek   Group: iGEM18_Bielefeld-CeBiTec   (2018-09-02)


OprC (TonB dependent copper transport porin, BBa_K2638200) with T7 promotor and RBS (BBa_K525998)

OprC is an outer membrane porin which binds and transports specifically copper. It belongs to the the superfamily of the TonB-dependent transporters. This variant of OprC was taken from Pseudomonas brassicacearum 3Re2-7 13, a not yet published strain.

This composite Part contains an T7 promotor and RBS so it can be expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 290
    Illegal NgoMIV site found at 621
    Illegal NgoMIV site found at 825
    Illegal NgoMIV site found at 1869
    Illegal NgoMIV site found at 2070
    Illegal AgeI site found at 284
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None