Part:BBa_K2637034:Design

KaiA cassette II with PGK1 Promoter and PGK1 Terminator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1412
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

PGK1 Promoter + KaiA + PGK1 Terminator. And KaiA has been optimized in yeast.

Source

PGK1P and PGK1T are the promoter and terminator of yeast. KaiA is from Synechococcus elongatus PCC7942.