Part:BBa_K2637030:Design

KaiA cassette with TEF1 Promoter and TEF1 Terminator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1349
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 266
  

Design Notes

TEF1 Promoter + KaiA + TEF1 Terminator. And KaiA has been optimized in yeast.

Source

TEF1P and TEF1T are the promoter and terminator of yeast. KaiA is from Synechococcus elongatus PCC7942.