Composite
Part:BBa_K2637024:Design
Designed by: Haoqian Zhao Group: iGEM18_Tianjin (2018-10-05)
AD-KaiC cassette with TDH3 Promoter and ADH1 Terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 366
Illegal BglII site found at 1725
Illegal BglII site found at 2232 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
TDH3 Promoter + AD + KaiC + ADH1 Terminator. And KaiC has been optimized in yeast.
Source
TDH3P and ADH1T are the promoter and terminator of yeast. Gal4 activation domain is from yeast genome and KaiC is from Synechococcus elongatus PCC7942.