Part:BBa_K2637022:Design

BD-CikA, Gal4 DNA binding domain fused with KaiB

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 590
  Illegal BamHI site found at 2650
  Illegal XhoI site found at 234
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 153
  

Design Notes

Gal4 DNA binding domain fused with CikA gene. CikA has been optimized in yeast.

Source

Gal4 DNA binding domain is from yeast genome and CikA is from Synechococcus elongatus PCC7942.