Part:BBa_K2636006

Aldh1, hmfH and hmfO operon under pBAD regulation

Aldh1^[1], hmfH^[2] and HmfO^[3] are three enzymes that allows oxidation of 5-(hydroxyméthyl)furfural ( HMF )into 2,5-Furandicarboxylic acid ( FDCA ). This operon regulated by pBAD promoter has been created in order to maximise the expression of protein that realise the oxidation of HMF into FDCA. These three enzymes use different pathways from HMF to FDCA with different intermediates so this operon could permit bypass of reaction intermediate accumulations.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 65
Illegal BamHI site found at 1766
Illegal XhoI site found at 475
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 393
Illegal NgoMIV site found at 711
Illegal NgoMIV site found at 802
Illegal NgoMIV site found at 1734
Illegal NgoMIV site found at 2428
Illegal NgoMIV site found at 2445
Illegal NgoMIV site found at 2466
Illegal AgeI site found at 1873
Illegal AgeI site found at 3220
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 754
Illegal BsaI.rc site found at 2355
Illegal BsaI.rc site found at 2878
Illegal SapI site found at 2523

References

↑ Hossain, G. S., Yuan, H., Li, J., Shin, H., Wang, M., Du, G., … Liu, L. (2017). crossm Metabolic Engineering of Raoultella, 83(1), 1–15.
↑ Koopman, F., Wierckx, N., de Winde, J. H., & Ruijssenaars, H. J. (2010). Efficient whole-cell biotransformation of 5-(hydroxymethyl)furfural into FDCA, 2,5-furandicarboxylic acid. Bioresource Technology, 101(16), 6291–6296. https://doi.org/10.1016/j.biortech.2010.03.050
↑ Dijkman, W. P., & Fraaije, M. W. (2014). Discovery and characterization of a 5-hydroxymethylfurfural oxidase from Methylovorus sp. strain MP688. Applied and Environmental Microbiology, 80(3), 1082–1090. https://doi.org/10.1128/AEM.03740-13

[1] Hossain, G. S., Yuan, H., Li, J., Shin, H., Wang, M., Du, G., … Liu, L. (2017). crossm Metabolic Engineering of Raoultella, 83(1), 1–15.

[2] Koopman, F., Wierckx, N., de Winde, J. H., & Ruijssenaars, H. J. (2010). Efficient whole-cell biotransformation of 5-(hydroxymethyl)furfural into FDCA, 2,5-furandicarboxylic acid. Bioresource Technology, 101(16), 6291–6296. https://doi.org/10.1016/j.biortech.2010.03.050

[3] Dijkman, W. P., & Fraaije, M. W. (2014). Discovery and characterization of a 5-hydroxymethylfurfural oxidase from Methylovorus sp. strain MP688. Applied and Environmental Microbiology, 80(3), 1082–1090. https://doi.org/10.1128/AEM.03740-13

[1]

[2]

[3]