Composite

Part:BBa_K2636005

Designed by: Sandro Papais   Group: iGEM18_Bordeaux   (2018-10-14)

hmfO under strong Promoter and strong RBS


HMF oxidase ( hmfO ) catalyses the oxidation of 5-(hydroxyméthyl)furfural ( HMF ) into 2,5-Furandicarboxylic acid ( FDCA ).[1] The regluation of this coding sequence by strong Promoter and RBS allows the transcription and translation at high level for E. coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1053
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. ↑ Dijkman, W. P., & Fraaije, M. W. (2014). Discovery and characterization of a 5-hydroxymethylfurfural oxidase from Methylovorus sp. strain MP688. Applied and Environmental Microbiology, 80(3), 1082–1090. https://doi.org/10.1128/AEM.03740-13
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