Regulatory

Part:BBa_K2633003:Design

Designed by: Julian Liber   Group: iGEM18_MichiganState   (2018-10-10)


proB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 39


Design Notes

The part has a single nucleotide single nucleotide change from T to A at position 82 to eliminate the restriction site XbaI.


Source

Originates from pJK_proB_eGFP (Bienick et al. 2014), with a single nucleotide change from T to A at position 82.

References

Bienick MS, Young KW, Klesmith JR, Detwiler EE, Tomek KJ, Whitehead TA. The interrelationship between promoter strength, gene expression, and growth rate. PLoS One. 2014 Oct 6;9(10):e109105.