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Composite
PcpcG2-PHB

Part:BBa_K2627005:Design

Designed by: Zhaoqin Zhang   Group: iGEM18_SDU-CHINA   (2018-10-06)


Gene cluster of phbcab controlled by PcpcG2-172 to produce PHB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 953
    Illegal PstI site found at 1526
    Illegal PstI site found at 2006
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 953
    Illegal PstI site found at 1526
    Illegal PstI site found at 2006
    Illegal NotI site found at 329
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 771
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 953
    Illegal PstI site found at 1526
    Illegal PstI site found at 2006
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 953
    Illegal PstI site found at 1526
    Illegal PstI site found at 2006
    Illegal NgoMIV site found at 382
    Illegal NgoMIV site found at 497
    Illegal NgoMIV site found at 731
    Illegal NgoMIV site found at 1043
    Illegal NgoMIV site found at 1322
    Illegal NgoMIV site found at 1735
    Illegal NgoMIV site found at 1802
    Illegal AgeI site found at 470
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3840


Design Notes

We did codon optimization.


Source

Chang, Y., et al., Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system. Microbial Cell Factories, 2016. 15(1). Nakajima, M., et al., Construction of a Miniaturized Chromatic Acclimation Sensor from Cyanobacteria with Reversed Response to a Light Signal. Scientific Reports, 2016. 6(1).


References