DNA

Part:BBa_K2625000:Design

Designed by: Cecilia Trivellin, Andrea Clausen Lind   Group: iGEM18_Chalmers-Gothenburg   (2018-10-08)


Alpha-Agglutin 2 (Aga2) fused to GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 974


Design Notes

A Kozac sequence at the 5' end of Aga2 has been added to improve chances of expression. A flexible linker (GGGS)x3 in between the proteins, TEF1 promoter and CYC1 terminator have also been added.



Source

Aga2: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GFP: Aequorea victoria (Jellyfish)

References

Tanaka, T., & Kondo, A. (2015). Cell-surface display of enzymes by the yeast Saccharomyces cerevisiae for synthetic biology. FEMS yeast research, 15(1), 1-9.