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DNA
Part:BBa_K2625000:Design
Designed by: Cecilia Trivellin, Andrea Clausen Lind Group: iGEM18_Chalmers-Gothenburg (2018-10-08)
Alpha-Agglutin 2 (Aga2) fused to GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 974
Design Notes
A Kozac sequence at the 5' end of Aga2 has been added to improve chances of expression. A flexible linker (GGGS)x3 in between the proteins, TEF1 promoter and CYC1 terminator have also been added.
Source
Aga2: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GFP: Aequorea victoria (Jellyfish)
References
Tanaka, T., & Kondo, A. (2015). Cell-surface display of enzymes by the yeast Saccharomyces cerevisiae for synthetic biology. FEMS yeast research, 15(1), 1-9.