Composite

Part:BBa_K2624007:Experience

Designed by: Huandi Xu   Group: iGEM18_CPU_CHINA   (2018-10-10)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2624007

We used this part to construct the "p-H*" plasmid and "p-H*-U6" plasmid, in order to verify the role of the RNA interference caused by pri-miRNA. Through qPCR experiment, it can be seen that pri-miRNA can successfully expressed and processed into miRNA.

20181017210154%21T--CPU_CHINA--hp-qidongzi.png

Figure. The expression of miRNA(MAP4K4)

"p-H*" contains "HULC--pri-miRNA" parts; "p-H*-U6" contains "HULC-pri-miRNA-U6-pSil" parts;

User Reviews

UNIQ7f547e011c7d34f7-partinfo-00000000-QINU UNIQ7f547e011c7d34f7-partinfo-00000001-QINU