Composite
NG4

Part:BBa_K2623024:Design

Designed by: Yunyun Hu   Group: iGEM18_XMU-China   (2018-10-02)


A non-GFP form of the BBa_K2623023


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 65
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

SpyTag is inserted to the C-termini or N-termini of the protein.


Source

Partly from E.coli's genomic sequence.


References