Coding
SAHS
Part:BBa_K2623007:Design
Designed by: Yingming Zhu Group: iGEM18_XMU-China (2018-09-28)
SAHS coding region
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 218
Illegal XhoI site found at 442 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 497
Illegal SapI.rc site found at 492
Design Notes
We got the DNA sequence of SAHS from DDBJ. And then we did some codon optimization to the DNA sequence for E.coli (strain K12).
Source
This part contains the coding region of the Hypsibius dujardini (Water bear) (Macrobiotus dujardini) SAHS gene.