Part:BBa_K2623003:Design

KaiC coding region

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 148
  Illegal AgeI site found at 859
  Illegal AgeI site found at 868
  Illegal AgeI site found at 874
  Illegal AgeI site found at 1198
  Illegal AgeI site found at 1225
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We got the protein sequence of KaiC from Uniprot. And then we did some codon optimization to the DNA sequence for E.coli (strain K12).

Source

This part contains the coding region of the Prochlorococcus (Synechococcus) elongatus PCC7942 KaiC gene.