Composite

Part:BBa_K2622045:Design

Designed by: Auksė Gaižauskaitė   Group: iGEM18_Vilnius-Lithuania   (2018-10-10)


BamE composite


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 106
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 508
    Illegal PstI site found at 106
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 456
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 106
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 106
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

- 

Warning: there is PstI restriction site inside the gene. Hoverer, it is still possible to clone by standart assemby.

Source

-

References