Composite

Part:BBa_K2622042:Experience

Designed by: Auksė Gaižauskaitė   Group: iGEM18_Vilnius-Lithuania   (2018-10-10)


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Applications of BBa_K2622042

Amplified gene sequence of BamB was ligated into a plasmid pET22b. pelB signal sequence directs BamB into periplasm. Expression was induced by 0,1mM IPTG. Bacteria cells were sonicated, membrane fraction was isolated by centrifugation and was further purified with Ni-NTA and gelfiltration. BamB was used in a folding assay, which showed that BamB and BamCDE lipoproteins can assemble BamA into the membrane with an efficiency higher than 50% in time range of only two hours. T--Vilnius-Lithuania--Fig7_BAM_compl.png T--Vilnius-Lithuania--Fig8_BAM_compl.png

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