Composite
Part:BBa_K2622033:Design
Designed by: Auksė Gaižauskaitė Group: iGEM18_Vilnius-Lithuania (2018-10-10)
His-igA
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 198
Illegal XbaI site found at 39
Illegal PstI site found at 181 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 198
Illegal NheI site found at 108
Illegal PstI site found at 181 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 198
Illegal BglII site found at 177
Illegal BamHI site found at 164
Illegal XhoI site found at 173 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 198
Illegal XbaI site found at 39
Illegal PstI site found at 181 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 198
Illegal XbaI site found at 39
Illegal PstI site found at 181
Illegal NgoMIV site found at 1041
Illegal AgeI site found at 816 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 20
Design Notes
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Source
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