Coding

Part:BBa_K2622024:Design

Designed by: Auksė Gaižauskaitė   Group: iGEM18_Vilnius-Lithuania   (2018-10-10)


SurA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 274
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 274
    Illegal NotI site found at 1321
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1023
    Illegal XhoI site found at 1330
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 274
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 274
    Illegal AgeI site found at 1134
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Warning: there is PstI restriction site inside the gene. Hoverer, it is still possible to clone by standart assemby.

Source

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References