RNA

Part:BBa_K2622007:Design

Designed by: Auksė Gaižauskaitė   Group: iGEM18_Vilnius-Lithuania   (2018-10-10)


gRNA for ribosome protein L12


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal SpeI site found at 52
    Illegal SpeI site found at 168
    Illegal PstI site found at 154
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 29
    Illegal SpeI site found at 52
    Illegal SpeI site found at 168
    Illegal PstI site found at 154
    Illegal NotI site found at 7
    Illegal NotI site found at 159
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal SpeI site found at 52
    Illegal SpeI site found at 168
    Illegal PstI site found at 154
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 52
    Illegal SpeI site found at 168
    Illegal PstI site found at 154
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

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References