Intermediate
Prom/RBS

Part:BBa_K262000:Design

Designed by: Leigh Armijo, Janice Cho, Nicholas Tschernia   Group: iGEM09_Nevada   (2009-10-07)

BBa_R0011 & BBa_B0034, IPTG-inducible promoter with Elowitz RBS.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Composite part designed for a highly efficient protein expression system. Combines the lacI regulated, IPTG-inducible promoter R0011 with the Elowitz RBS B0034.

This specific composite is within the pSB3C5 low to medium copy BioBrick standard vector.

Source

BBa_R0011 was used from the Spring 2009 Kit Plate 1 Distribution (well 6G)

BBa_B0034 was used from the Spring 2009 Kit Plate 1 Distribution (well 2M)

References