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Part:BBa_K2618001:Design

Designed by: Tim (2018 HKU team)   Group: iGEM18_HK_HCY_LFC   (2018-09-02)


ssDNA for DNA tweezers assembly (Strand 2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101
    Illegal SapI site found at 242


Design Notes

Since this part is designed to be reverse transcribed into a single-strand DNA, so a HIV reverse transcriptase binding site is added.


Validation of the plasmids with the DNA inserts amplified by PCR. The band surrounded in red box is the part of the DNA insert amplified.

PAGE_for_the_validation_of_plasmid.png


Agar plate showing the transformed colonies with BBa_K2618001

BBa_K2618001_plate.PNG

Source

It is newly designed.

References