Coding
Part:BBa_K2617004:Design
Designed by: Yansong Wang Group: iGEM18_UESTC-China (2018-09-30)
Activated Escherichia coli formic acid lyase (FHL) system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1323
Illegal NgoMIV site found at 1896
Illegal AgeI site found at 1375 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
To get a good expression of this gene in E. coli, we did codon optimization before DNA synthesis
Source
The source of the part will be its sequence which was retrieved from GenBank.