Coding

Part:BBa_K2617000:Design

Designed by: Yetao Zou   Group: iGEM18_UESTC-China   (2018-09-30)


Xyn10D-Fae1A：Bifunctional xylanase/ferulic acid esterase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 534
    Illegal AgeI site found at 381
    Illegal AgeI site found at 628
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1236


Design Notes

To get a good expression of this gene in E. coli, we did codon optimization before DNA synthesis.


Source

The source of the part will be its sequence which was retrieved from GenBank.

References