Device
miniToe te

Part:BBa_K2615009:Design

Designed by: Anyi Li   Group: iGEM18_OUC-China   (2018-09-07)


The miniToe family test system No.2.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 197


Design Notes

The Csy4 binding site is a mutant of the wild type. The sequence of 22nt hairpin is changed from GTTCACTGCCGTATAGGCAGCT to GTTCACGGCCGTATAGGCCGCT.

Source

Science Museum No.105,College of Marine Life Sciences, Ocean University of China

References