RNA
Part:BBa_K2611009:Design
Designed by: Yumei Yang Group: iGEM18_SCU-China (2018-10-17)
sgRNA (J23100)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We need to make sure that this sgRNA sequence can bind to the reverse sequence of J23100 promoter, and at the meantime, it cannot bind to any other DNA sequence of E.coli plasmids nor genome.
Source
We designed the sgRNA sequence by ourselves and had it synthesized by Tsingke Biological Technology.co.