RNA
Part:BBa_K2611009
Designed by: Yumei Yang Group: iGEM18_SCU-China (2018-10-17)
sgRNA (J23100)
This part contains a sgRNA generator with a sgRNA sequence that can specifically bind to the reverse sequence of J23100 promoter. Our project is to use sgRNA sequences to programmably regulate the expression of different genes in E.coli genome, with methods like AND gates and OR gates. So we need to find several different sgRNA sequences that can bind to different parts of one same gene. This part is one of such sgRNA sequences we designed that binds to J23100 promoter, so, with dCas 9 protein, it can regulate the expression of genes with J23100 promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
biology | |
negative_regulators |