Reporter
Part:BBa_K2611007
Designed by: Yibing Tao Group: iGEM18_SCU-China (2018-10-07)
T7 promoter-RFP-J23100-GFP
This year, our team aims to construct a bistable gene switch to verify the function and the orthogonality of our dCas9/ sgRNA regulatory system. This plasmid is supposed to function as the reporter of our final bistable switch circuits. T7 promoter-sgRNA (J23100)(BBa_K2611004) and T7 promoter-sgRNA (BBa_K2611005), which designed to express the specific sgRNA and help the dCas9 protein to target specific sequence on the reporter plasmid (such as the J23100 promoter and the coding sequence of GFP), are also newly submitted to the registry by our team this year.
File:Bistable switch circuit.png
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 907
Illegal NheI site found at 930 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 604
Illegal AgeI site found at 716 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1605
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Categories
Parameters
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