Reporter

Part:BBa_K2611007

Designed by: Yibing Tao   Group: iGEM18_SCU-China   (2018-10-07)


T7 promoter-RFP-J23100-GFP

This year, our team aims to construct a bistable gene switch to verify the function and the orthogonality of our dCas9/ sgRNA regulatory system. This plasmid is supposed to function as the reporter of our final bistable switch circuits. T7 promoter-sgRNA (J23100)(BBa_K2611004) and T7 promoter-sgRNA (BBa_K2611005), which designed to express the specific sgRNA and help the dCas9 protein to target specific sequence on the reporter plasmid (such as the J23100 promoter and the coding sequence of GFP), are also newly submitted to the registry by our team this year.

File:Bistable switch circuit.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 907
    Illegal NheI site found at 930
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 604
    Illegal AgeI site found at 716
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1605


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Categories
Parameters
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