Reporter
Part:BBa_K2611001:Design
Designed by: Changhe Li Group: iGEM18_SCU-China (2018-10-17)
spacer J23101-GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 30
Illegal NheI site found at 53 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 728
Design Notes
This part should be used with sgRNA(spacer J23101-GFP)(BBa_K2611000).
Source
The spacer sequence is selected from a reported sequence in which the function of this spacer has been proved. And the backbone we used is from BBa_J364000.
Referance:Bikard, D., Jiang, W., Samai, P., Hochschild, A., Zhang, F., & Marraffini, L. A. (2013). Programmable repression and activation of bacterial gene expression using an engineered crispr-cas system. Nucleic Acids Research, 41(15), 7429-7437.
References
Bikard, D., Jiang, W., Samai, P., Hochschild, A., Zhang, F., & Marraffini, L. A. (2013). Programmable repression and activation of bacterial gene expression using an engineered crispr-cas system. Nucleic Acids Research, 41(15), 7429-7437.