Regulatory

Part:BBa_K2605003:Design

Designed by: Kaitlin Schaaf   Group: iGEM18_Calgary   (2018-10-09)


A2UCOE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 73
    Illegal XhoI site found at 134
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part contains a 5' MfeI restriction site and 3' NsiI restriction site so it can be used within our Multiple-Cloning Site flanked by FRT sites Part BBa_K2605002 system.


Source

The 8kb UCOE from the human HNRPA2B1-CBX3 (RNP/A2UCOE) locus can be shortened down to a core 1.2kb fragment that functions as effectively as the whole fragment. The sequence for this 1.2kb fragment was taken from literature (Zhang et al., 2010).

References

Zhang, F., Frost, A.R., Blundell, M.P., Bales, O., Antoniou, M.N., Thrasher, A.J. (2010). A ubiquitous chromatin opening element (UCOE) confers resistance to DNA methylation-mediated silencing of lentiviral vectors. Molecular Therapy, 18(9), 1640-49.