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2018 iGEM team Linkoping Sweden

2018 iGEM team Linkoping Sweden validated this part.

Validation of GFP fluorescence

The experimental setup: Bacteria were grown until an OD600 of 0.2-0.4 in LB-medium and 25 ug/ml chloramphenicol. They were then placed in an 96-well plate in quadruplicates. The OD was measured in the start and the end of the experimental time. Fluorescence was measured every 15 minutes for 16 hours and the temperature was set to 37°C. The values shown in Figure. 1 are the fluorescence intensity divided by the start OD. Excitation was measured at 485 nm and emission at 520 nm.

Figure 1. GFP intensity over 16 hours. Error bars represent Standard Deviation. Total time is 16 hours and fluorescence was measured every 15 minutes.