Part:BBa_K2602021:Experience
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2018 iGEM team Linkoping Sweden
2018 iGEM team Linkoping Sweden validated this part.
Validation of GFP fluorescence
The experimental setup: Bacteria were grown until an OD600 of 0.2-0.4 in LB-medium and 25 ug/ml chloramphenicol. They were then placed in an 96-well plate in quadruplicates. The OD was measured at the beginning and at the end of the experimental time. Fluorescence was measured every 15 minutes for 16 hours and the temperature was set to 37°C. The values shown in Figure. 1 are the fluorescence intensity divided by the starting OD. Excitation was measured at 485 nm and emission at 520 nm.
