Plasmid_Backbone

Part:BBa_K260010:Design

Designed by: Kaj Bernhardt   Group: iGEM09_BIOTEC_Dresden   (2009-10-18)

pMA-BsdR-@CC2FosM


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3076
    Illegal NheI site found at 3014
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3082
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3076
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3076
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3076
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3091
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

The 2x 70 bp homologies were designed to delete 100 bp of pCC2FosM (BBa_K260000). The insertion site on pCC2FosM was chosen to be upstream of MECP2 exon 1 but still with more than 10 kb of MECP genomic sequence upstream.


Source

Geneart plasmid pMA, with codon-optimised blasticidin resistance cassette (BsdR) and 2x 70 bp from pCC2FosM (BBa_K260000).

References