Plasmid_Backbone

Part:BBa_K260009:Design

Designed by: Kaj Bernhardt   Group: iGEM09_BIOTEC_Dresden   (2009-10-18)

pMA-RQ-@TetFlp


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 67
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2578
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2584
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2578
    Illegal XhoI site found at 61
    Illegal XhoI site found at 2572
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2578
    Illegal suffix found at 2
    Illegal XbaI site found at 67
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2578
    Plasmid lacks a suffix.
    Illegal XbaI site found at 67
    Illegal XbaI site found at 2593
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

The 2x 70 bp homologies were designed to allow "pseudo-cloning" of a BioBrick from the present backbone pMA-RQ-@TetFlp to pTetFlp BBa_K260002 via recombineering using the PvuII fragment containing the BioBrick, where BioBrick prefix and suffix are kept untouched.


Source

Geneart plasmid pMA-RQ, with 2x 70 bp from pTetFlp BBa_K260002.

References