Part:BBa_K260009

pMA-RQ-@TetFlp

This plasmid backbone is based on pMA-RQ from Geneart. It has BioBrick prefix and suffix flanked on both sides by 70 bp of homology to pTetFlp (BBa_K260002) for tranfer of the contained BioBrick part by recombineering, for which the BioBrick needs to be excised with PvuII from the backbone.

This plasmid backbone is available with BioBrick P_F3_zeoR_F3_RFP (BBa_K260017) depicted below. The product of the above-described recombineering reaction BBa_K260002-BBa_K260017 is also available.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 67
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2578
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 2584
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2578
  Illegal XhoI site found at 61
  Illegal XhoI site found at 2572
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 2578
  Illegal suffix found at 2
  Illegal XbaI site found at 67
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 2578
  Plasmid lacks a suffix.
  Illegal XbaI site found at 67
  Illegal XbaI site found at 2593
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.