Composite

Part:BBa_K2598032:Design

Designed by: Matthew Gao   Group: iGEM18_UCAS-China   (2018-09-25)


gusA under K1F-inducible promoter pK1F


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

The subparts are provided by Christopher A Voigt and is assembled via Gibson Assembly.

References

Fernandez-Rodriguez, J., Moser, F., Song, M. & Voigt, C. A. Engineering RGB color vision into Escherichia coli. Nature Chemical Biology 13, 706-708 (2017)

Segall-Shapiro, T. H., Meyer, A. J., Ellington, A. D., Sontag, E. D. & Voigt, C. A. A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol 10, 742, (2014).

Kye-Won Kim, V. R. F., Laurence B. Davin, and Norman G. Lewis. in Arabidopsis Protocols Vol. 323 Methods in Molecular Biology (ed J. Salinas and J.J. Sanches-Serrano) Ch. 22, 263-273 (Humana Press, 2006).