Part:BBa_K2598013:Design
T7 RNAP sigma fragment CGG under CcaR-regulated promoter PcpcG2
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 256
Illegal NgoMIV site found at 265
Illegal NgoMIV site found at 880 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
An insulator BydvJ is added to render the output irrespective of the identity of the input promoter. A strong synthetic is added to avoid recombination.
Source
PcpcG2 is from the genome of Synechocystis PCC6803 CGG and insulator is provided by Jesus Fernandez-rodriguez et al terminator is synthesized
References
Fernandez-Rodriguez, J., Moser, F., Song, M. & Voigt, C. A. Engineering RGB color vision into Escherichia coli. Nature Chemical Biology 13, 706-708 (2017)
Schmidl, S. R., Sheth, R. U., Wu, A. & Tabor, J. J. Refactoring and optimization of light-switchable Escherichia coli two-component systems. ACS Synth Biol 3, 820-831, (2014).
Lou, C., Stanton, B., Chen, Y. J., Munsky, B. & Voigt, C. A. Ribozyme-based insulator parts buffer synthetic circuits from genetic context. Nat Biotechnol 30, 1137-1142, (2012).
Segall-Shapiro, T. H., Meyer, A. J., Ellington, A. D., Sontag, E. D. & Voigt, C. A. A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol 10, 742, (2014).