Device

Part:BBa_K2598001:Design

Designed by: Xu Zepeng   Group: iGEM18_UCAS-China   (2018-09-18)


T7 RNAP core fragment under constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1860
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We use this part along with sigma fragments to accurately control the outputs


Source

This is designed based on T7 RNAP.

References

Fernandez-Rodriguez, J., Moser, F., Song, M. & Voigt, C. A. Engineering RGB color vision into Escherichia coli. Nature Chemical Biology 13, 706-708 (2017)

Chen, Y. J. et al. Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Methods 10, 659-664, (2013)